DNA Duplication

DNA Duplication (or replication) is the process involving the separation (unzipping) the double stranded DNA molecule into two stands, each of which serves as a template to assemble a new complementary strand of DNA. The process is as follows: So the order of enzymes goes Helicase, Topoisomerase, Primase, DNA Polymerase, DNA Ligase, or HTPPL.
 * 1) Helicase unwinds the DNA, producing a replication fork
 * 2) SSB (Single Stranded DNA Binding Proteins): Keep DNA from reattaching
 * 3) Topoisomerase: Removes twists and knots ahead of the replication fork that form in the double stranded template as a result of the unwinding induced by helicase.
 * 4) Primase: Primes the DNA for replication; starts an RNA chain that will eventually be replaced by DNA Nucleotides.
 * 5) DNA Polymerase I, II, III: Takes RNA primer out, replaces with DNA, adds nucleotides.
 * 6) DNA Ligase: Seals the Okazaki fragments in the lagging strand.